A requirement for MCM7 and Cdc45 in chromosome unwinding during eukaryotic DNA replication
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چکیده
منابع مشابه
A requirement for MCM7 and Cdc45 in chromosome unwinding during eukaryotic DNA replication.
In vertebrates, MCM2-7 and Cdc45 are required for DNA replication initiation, but it is unknown whether they are also required for elongation, as in yeast. Moreover, although MCM2-7 is a prime candidate for the eukaryotic replicative DNA helicase, a demonstration that MCM2-7 unwinds DNA during replication is lacking. Here, we use Xenopus egg extracts to investigate the roles of MCM7 and Cdc45 i...
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Miniature chromosome maintenance (MCM) proteins were initially identified from autonomously replicating sequence in Saccharomyces cerevisiae. Mutations of some of these proteins such as MCM7 or MCM3 in yeast result in loss of the large chunk of yeast chromosomes. MCM7 cDNA encodes a 543-amino acid protein and is ubiquitously expressed in all tissues. Initiation of DNA replication is a complex p...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2004
ISSN: 0261-4189,1460-2075
DOI: 10.1038/sj.emboj.7600369